Database of Primer Scientific Names (DPSN) contains 177 SSU primers and 38 LSU primers for amplicon sequencing. The naming rule of short name is now improved to have five basic components: a character for target gene - a character for taxonomic group, 5’ position on the sense strand, version and direction. 

Motivation: Amplicon sequencing is the most widely used sequencing methods to evaluate microbial diversity in virtually all environments. To amplify amplicon region, primers is required in amplicon sequencing. probeBase is a curated database of rRNA-targeted probes and primers. However, 63.58% of the primers in probeBase have problems in short name, full name and/or position. Furthermore, the naming rule of short name leads to ambiguity and needs improvement.

Usage: There are 6 fields in the output: corrected new short name, corrected full name，short name (original in probeBase)，full name (original in probeBase), sequence and 5’position. Users can use any content in one of these fields as the searching key. Meanwhile, the database supports fussy search, any match of substrings will be reported. 

Citation: the related article is under submission and will be update soon.

Primer submission: submissions from researchers for new primers are welcome and encouraged. Please provide the correct full name and the full sequence of the new primer through email.

Contact: Yuxiang Tan, Email：36410463@qq.com

News:

2020.01.23: We updated the target gene in short name convention. 38 LSU primers were also added.

2019.10.01: Four new primers were updated into DPSN.

2019.04.04: DPSN was firstly online.